The Plasmid Miniprep Kit provides a simple, fast and cost-effective method to purify plasmid DNAs from bacterial culture, especially useful for low-copy-number plasmid miniprep or applications that amount of DNA needed is about 10-200 µg. The kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. The yield of the high-copy plasmid from this kit is about 10-20µg. Plasmid DNAs purified from this kit can be used directly for downstream applications such as transfection, cloning, sequencing, as well as in vivo applications.