HBSS (Hanks' Balanced Salt Solution) is used for a variety of cell culture applications, including washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. HBSS without calcium and magnesium is required for rinsing chelators from the culture before cell dissociation. Hygia's balanced salt solutions are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of HBSS solutions are subjected to sterility test, pH, osmolality and endotoxin.