Opt for a PVDF membrane plate with high affinity binding
Capture antibodies with highest affinity yield best results
Optimize conditions empirically, including cell density, stimulus/mitogen, and kinetics
Description
This Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates is a pre-prepared kit for detecting IL-33R signaling. It comes with a pre-titrated, high-quality capture antibody, a biotin-conjugated detection antibody, and other necessary components. While optimized for ELISPOT assays, it's not recommended for that specific use.