ACTaq Blue / Red Hot-Start Master mix is complete "ready-to-go" heat–activated 2X reaction mix which only requires the consumer researcher to add water, template and primers, and then pre-heat to 95°C for reaction activation. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of Hot-Start Taq enzyme, it goes further by including all of the "ingredients" required for thermal cycling and blue tracking and loading dye.