ACTi High Fidelity DNA Polymerase, originating from Pyro coccus furious with molecular weight of 90KDa, is a thermos stable enzyme and exhibits the lower error rate of DNA amplification. For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300µM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1. ACTi High Fidelity DNA Polymerase generates blunt-ended fragment.