Ice-free Freeze Box is a new type of energy-saving, safe, and has no additives. The Freezer Box requires no electricity (other than freezer), no ice required for low temperature protection for biological samples. Ice core aluminum alloy shell with high thermal conductivity of the alloy module to ensure that the sample can be quickly cooled, the temperature difference between the sample holes less than 0. 1°C, maintains a uniform temperature between the samples. The Freeze Box is ice-free, lightweight, easy to carry, and ideal for collection of biological samples, it also can also be used in helping to preserve the unstable nucleic acid samples. Its compact design and ice-free technology make it ideal for use in a tissue culture incubator, bio-safety cabinet and any other application with limited space. The Freeze Box can be used with different ice cores, and a variety of thermal test tube modules for usage with different samples and temperature.